Classification of Salmonella enterica of the (Para-)Typhoid Fever Group by Fourier-Transform Infrared (FTIR) Spectroscopy

Typhoidal and para-typhoidal Salmonella are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious resource-demanding serotyping essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing simple bacterial typing technology. In this study, we assessed the discriminatory power FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, Germany), for rapid reliable identification biochemically confirmed typhoid paratyphoid fever-associated isolates. total, 359 isolates, comprising 30 S. Typhi, 23 Paratyphi A, B, 7 C, respectively other phylogenetically closely related serovars belonging serogroups O:2, O:4, O:7 O:9 were tested. The strains derived from clinical, environmental food samples collected at different European sites. Applying artificial neural networks, specific automated classifiers built discriminate typhoidal non-typhoidal within each four serogroups. accuracy was 99.9%, 87.0%, 99.5% 99.0% B respectively. promising tool fast detection Salmonella. Hence, biotyping may serve as suitable alternative conventional approaches surveillance diagnostic purposes.